Assessment of zygosity and nitrogen used efficacy in Mekongga transgenic rice
نویسندگان
چکیده
Abstract. Aini MQ, Suharsono, Apriana A, Sisharmini Santoso TJ, Trijatmiko KR. 2022. Assessment of zygosity and nitrogen used efficacy in Mekongga transgenic rice. Biodiversitas 23: 4040-4046. Nitrogen (N) is a vital macronutrient that can be limiting factor for rice plant growth. Nitrogen-efficient plants assembled by modifying nitrogen-related metabolic pathways to improve Use Efficiency (NUE) plants. T0 generation containing the LeAlaAT gene was obtained. In research, test desirable ensure integration inheritance stability transgene. This study aimed assess use two lines, namely M41 M50. research involved three main activities, i.e. detection flanking sequence TAIL-PCR method, T1 generation, T2 lines response different N fertilizer rates 0, 60, 90, 120 kg ha?1. Zygosity analysis showed six out 14 13 M50 were homozygous The had higher tiller numbers, grain biomass compared non-transgenic ones under same level. notable discrepancies also recognized absorption, agronomical, NUE studied
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ژورنال
عنوان ژورنال: Biodiversitas
سال: 2022
ISSN: ['1412-033X', '2085-4722']
DOI: https://doi.org/10.13057/biodiv/d230823